Curriculum vitae

 

Names (first, middle, second):

Oleg Victorovich Mosin.

 

Country of birth

Russia

 

Title:

Candidate of Science (Chemistry), Ph D.

 

Qualification:

 

Engineer of Technology.

 

Work:

 

Part-time lecturer at Moscow State University of Food Production, 125080, Moscow, Volokolamskoet Shosse, 11, telepnone 158-03-71.

 

Scientific interests:

 

Application of various microbial objects for the preparation of cell compounds including proteins, amino acids, carbohydrates and nucleosides labeled with stable isotopes 2H, 13C, 15N;

Metabolism of stable isotopes;

Biological adaptation to heavy water and effects of heavy water on living objects;

Adaptation to heavy water;

Molecular evolution.

 

Research:

 

Preparation of isotopically labelled cell compounds with using various microbial objects;

Biological adaptation to heavy water, and biological effects of heavy water on organism;

Preparation of [2H]-and [13C]-amino acids with high levels of enrichment via bioconvertion of C2H3O2H/13CH3OH and heavy water in methylotrophs Brevibacterium methylicum and Methylobacillus flagellatum;

The studying of amino acids biosynthesis by facultative methylotroph Brevibacterium methylicum in the presence of heavy water;

Biosynthesis of bacteriorhodopsin labeled with deuterium on the residues of aromatic amino acids [2,3,4,5,6-2H5]phenylalanine, [3,5-2H2]tyrosine and [2,4,5,6,7-2H5]tryptophan; the growth of Bacillus subtilis and biosynthesis of inosine on highly deuterated media, containing heavy water and deuterated biomass of methylotrophic bacterium B. methylicum;

Electron impact masss-pectrometry and NMR of isotopically labeled compounds;

 

Scientific Awards:

 

Winner of the President Prize, nominated by the decree of President of Russia Federation from 6.11. 1993 for 1 613-pт, from 27.01.94 171 for distinguished successes in studies and scientific research.

 

 

Details of Relevant Experience:

 

12.09. 2006-present time. Part-time lecturer at Moscow State University of Food Production, 125080, Moscow, Volokolamskoet Shosse, 11, 7 (499) 158 03 71, mgupp@mgupp.ru.

21.07.1996-2000. Junior research worker, Biotechnology Department, M. V.Lomonosov State Academy of Fine Chemical Technology, Prospekt Vernadskogo 86, Moscow B-571, 117571, faxmile (495) 434 80 64, tel (495) 434 94 74, email mitht@mitht.ru

14.09.1993-21.04.1996. Engineer for technical assistance, Department of Foreing languages, M. V. Lomonosov State Academy of Fine Chemical Technology, Prospekt Vernadskogo 86, Moscow B-571, 117571, Russia, tel (095) 246 47 81.

 

Academic history:

 

1.12.1992-3.06.1996. Candidate of Science (Chemistry), diploma KT N 022522,

Biotechnology Department, M. V. Lomonosov State Academy of Fine Chemical Technology, The dissertation thesis supported (the combined research was done under the advisorship of a correspondent member of Russian Academy of Medical Sciences, professor Vitalyi I. Shvets and a leader Scientist of the State Scientific Centre of Russian Federation GNIIGENETIKA Dr. Dmitry A. Skladnev): The developing of various biotechnological methods for the preparation of proteins, amino acids and nucleosides labeled with stable isotopes - 2H and 13C with highly levels of isotopic enrichment.

1.09.1984-10.02.1992. Engineer of Technology, diploma ЦВ N 237951, Biotechnology Department, M.V. Lomonosov State Academy of Fine Chemical Technology, The diploma thesis supported (the research was done under the advisorship of a leader Scientist of the State Scientific Centre of Russian Federation GNIIGENETIKA Dr. Dmitry A. Skladnev): The study of bioconvertion of isotopically labeled low wheighted substrates (C2H3O2H and 2H2O ) in methylotrophic bacteria.

1.09.1974-26.06.1984. The State High School of Biology N 917, ulitza Amurskaja 20, Moscow, 107241, diploma sertificate АБ 1 531567.

 

Technical Skills:

 

Methods of biological and enzymatic investigation, cell growing, mutagenise and strain construction, analytical and separation methods of chromatography LC (HPLC), spectral methods including nuclear magnetic resonance and mass spectrometry.

 

Academic References and scientific people who know me.

 

1. Correspondent member of Russian Academy of Medical Sciences, professor Vitalyi I. Shvets, Doctor of Science (Chemistry), head of Biotechnology Department and prorector of M. V. Lomonosov State Academy of Fine Chemical Technology, Prospekt Vernadskogo 86, Moscow B-571, 117571, Russia, tel (095) 437 98 57; (095) 437 35 27; fax (095) 430 79 83.

2. Professor Alexander M. Yurkevich, Doctor of Science (Chemistry), Biotechnology Department of M. V. Lomonosov State Academy of Fine Chemical Technology, Prospekt Vernadskogo 86, Moscow B-571, 117571, Russia, tel (095) 437 98 57; (095) 437 35 27; fax (095) 430 79 83.

3. Dr. Dmitry A. Skladnev, Candidate of Science (Biology), Leader Scientist, State Scientific Centre of Russian Federation GNIIGENETIKA, Doroznyi proezd, 1, Moscow, 113545, Russia, tel (095) 315 37 10; (095) 314 37 47; fax (095) 315 05 01; telex 411718 Genom SU; E-mail boss@vnigen.msk.SU.

4. Dr. Ignat Ignatov, Holder of World Prizes for Alternative Medicine and Biophysics Head of SRCMB, SCIENTIFIC RESEARCH CENTER OF MEDICAL, BIOPHYSICS /SRCMB/Sofia, Bulgaria, Town of Teteven,  bl. Obedinenie, entr.V, apt.1 Tel. +359 – 678 – 33 44 Mail address: Sofia 1111, 32 Nikolai Kopernik st., apt. 6, Tel.  +359 – 2 –872 99 19, +359889 – 63 74 20, E-mail: mbioph@dir.bg, www.medicalbiophysics.dir.bg

 

List of main publications:

 

1. Mosin O. V., Karnaukhova E. N., Skladnev D. A., et al. Biosynthetic preparation of deuterated phenylalanine by methylotroph Brevibacterium methylicum. // Biotechnologija. 1993. N.9. P. 16-20.

2. Еgorova T. A., Mosin O. V., Shvets V. I., et al. Preparative separation of N-benzylcarboxy-derivatives of amino acids obtained from protein hydrolysates. // Biotechnologija. 1993. N.8. P. 21-25. 

3. Bekker G. D., and Mosin O. V. Amino acids labeled with stable isotopes. Preparation and mass spectrometric control. in: 4th All-Russian Students Scientific Conference. Problems of Abstract and Applied Chemistry. 20-22 April. 1994. Ural State University. Ekaterinburg. P.127.

4. Mosin O. V., Karnaukhova E. N., Skladnev D. A., Tsygankov Y. D. A strain of facultative methylotrophic bacterium Brevibacterium methylicum - producent of phenylalanine, 1993, Russian patent N 93055824. Positive solution  ¹ 055610 from 17.11.1995 for obtaining Russian patent.

5. Kazarinova L. A,  Mironov A. S., Mosin O. V., Skladnev D. A., Yurkevich A. M. A method for the preparation of nucleosides and nucleotides labeled with deuterium with high levels of isotopic enrichment, 1995, Application for Russian patent N 95118778.

6. Karnaukhova E. N., Mosin O. V., and Reshetova O. S. Biosynthetic production of stable isotope labeled amino acids using methylotroph Methylobacillus flagellatum. // Amino Acids. 1993. V.5. N.1.P.125.

7. Mosin O. V., Karnaukhova E. N., Pshenichnikova A. B., Reshetova O. S. Electron impact spectrometry in bioanalysis of stable isotope labeled bacteriorhodopsin. in: Sixth International Conference on Retinal Proteins. 19-24 June 1994. Leiden. The Netherlands. P.115.

8. Mosin O. V., Karnaukhova E. N., Skladnev D. A. Application of methylotrophic bacteria for the preparation of stable isotope labeled amino acids. in: 7th International Symposium on the Genetics of Industrial Microorganisms. 26 June 1July  1994. Quebec. Canada. P. 163.

9. Matveev A. V., Mosin O. V., Skladnev D. A., and Yurkevich A. M. Methylotrophic adaptation to highly deuterated substrates. in: 8th International Symposium on Microbial Growth on C1 Compounds. 27 August-1 September 1995. San Diego. U.S.A. P. 79.

10. Mosin O. V., Karnaukhova E. N., and Skladnev D. A. Preparation of  2H-and 13C-labeled amino acids via bioconvertion of C1-substrates. in: 8th International Symposium on Microbial Growth on C1 Compounds. 27 August-1 September 1995. San Diego. U.S.A. P. 80.

11. Shvets V. I., Yurkevich A. M., Mosin O. V., Skladnev D. A. Preparation of deuterated inosine suitable for biomedical application. // Karadeniz Journal of Medical Sciences. 1995. V.8. N 4. P.231-232.   

12. Mosin O. V., Skladnev D. A., Egorova T. A., Yurkevich A. M., Shvets V. I. The studying of amino acid biosynthesis by facultative methylotroph Brevibacterium methylicum on media containing heavy water. // Biotechnologija. N 3. 1996. P. 3-12.

13. Mosin O. V., Egorova T. A., Chebotaev . B., Skladnev D. A., Yurkevich A. M., Shvets V. I. Preparation of bacteriorhodopsin labeled with deuterium on the residues of aromatic amino acids phenylalanine, tyrosine and triptophan. // Biotechnologija. 1996. N 4. P. 27-34.

14. Mosin O. V., Kazarinova L. A., Preobrazenskaya K. A., Skladnev D. A., Yurkevich A. M., Shvets V. I. The growth of the bacterium Bacillus subtilis and biosynthesis of inosine on highly deuterated media. // Biotechnologija. 1996. N 4. P. 19-26.

15. Skladnev D. A., Mosin O. V., Egorova T. A., Eremin S. V., Shvets V. I. Methylotrophic bacteria as sourses of 2Hand 13C-labeled amino acids. // Biotechnologija. N 5. 1996. P. 14-22.

 

16. Mosin O. B., Skladnev D. A., Egorova T. A., Shvets V. I. Mass spectrometric evaluation of 2H-and 13C enrichment of amino acids molecules obtained from bacterial objects // Bioorganicheskaja khimia. 1996. V. 22. N 10-11. P. 861-874.

17. Mosin O. B., Skladnev D. A., Egorova T. A., Shvets V. I. The methods for the preparation of proteins and amino acids labeled with stable isotopes 2H, 13C, 15N, 18O // Biotechnologija. N 10. P. 1-17.

18. Mosin O. B., Skladnev D. A., Egorova T. A., Shvets V. I. Biosynthesis of deuterated bacteriorhodopsin by Halobacterium halobium. // 7th International Conference “New developments in biotechnology”. 15-20 May 1996. Pushino. Russian Federation. P. 95.

19. Mosin O. B., Skladnev D. A. Shvets V. I Investigation of process of physiological abaptation of methylotrophic bacteria to 2H2O. // Conference “Autotrophic microorganisms”. Moscow.  17-20 April 1996. P. 113

20. Mosin O. V., Skladnev D. A., Egorova T. A., Shvets V. I. Biotechnological potential of methylotrophs for the preparation of deuterated amino acids. // 8th International Congress of Bacteriology and Applied Microbiology Division. Jerusalem. Israel. August 18-23. 1996. P. 56

21. Kazarinova L. A., Preobrazhenskaja E. S.,Mosin O. B., Skladnev D. A., Yurkevich A. M., Shvets V. I. Production of deuterated inosine by Bacillus subtilis. // 8th International Congress of Bacteriology and Applied Microbiology Division. Jerusalem. Israel. August 18-23. 1996. P. 82

22. Mosin O. V., Skladnev D. A., Egorova T. A., Shvets V. I. The methods for preparations of 2H-labeled proteins, amino acids and nucleosides. // Conference in commemoration of Preobrazensky N. A., M. V. Lomonosov state academy of fine chemical technology. November 1996. P. 89.

23. Mosin O. V., Skladnev D. A., Shvets V. I. Introduction of ino [2,3,4,5,6 -2H]phenylalanine, [3,5 -2H]tyrosine and [2,4,5,6,7]tryptophane into molecule of bacteriorhodopsine of Halobacterium halobium. // Prikladnayia biokhimija microbiologija. 1999, N1, PP. 34-45.

24. Mosin O. V., Skladnev D. A., Shvets V. I. Biosynthesis of 2 H-labeled phenylalanine by a new mutant of RuMP facultive methylotroph Brevibacterium methylicum // Biosience, biotechnolody and bioengineering, 62, N 2, PP. 225-229, 1997.

 

Additional information about me:

 

viperson.ru/wind.php?ID=620740

www.famous-scientists.ru/3634/

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www.wiw-rf.ru/memberPerson/42346